Assembling a dual purpose TaqMan-based panel of single-nucleotide polymorphism markers in rainbow trout and steelhead (Oncorhynchus mykiss) for association mapping and population genetics analysis

作者：Hansen Mette H H^*; Young Sewall; Jorgensen Hanne B H; Pascal Carita; Henryon Mark; Seeb James

来源：Molecular Ecology Resources, 2011, 11: 67-70.

DOI：10.1111/j.1755-0998.2010.02978.x

• association mapping
• disease resistance
• population genetics
• rainbow trout
• SNP
• steelhead

We establish a TaqMan-based assay panel for genotyping single-nucleotide polymorphisms in rainbow trout and steelhead (Oncorhynchus mykiss). We develop 22 novel single-nucleotide polymorphism markers based on new steelhead sequence data and on assays from sister taxa. Additionally, we adapt 154 previously developed markers to the TaqMan platform. At the beginning of this study, 59 SNPs with TaqMan assays were available to the scientific community. By adding 176 additional TaqMan assays to this number, we greatly expand the biological applications of TaqMan genotyping within both population genetics and quantitative genetics.

• 出版日期2011-3