Digital image analysis of membrane connectivity is a robust measure of HER2 immunostains

作者：Brugmann Anja^*; Eld Mikkel; Lelkaitis Giedrius; Nielsen Soren; Grunkin Michael; Hansen Johan D; Foged Niels T; Vyberg Mogens

来源：Breast Cancer Research and Treatment, 2012, 132(1): 41-49.

DOI：10.1007/s10549-011-1514-2

• Breast cancer
• HER2
• Image analysis
• Immunohistochemistry
• FISH
• Tissue microarray

The purpose of this study was to develop and validate a new software, HER2-CONNECTTM, for digital image analysis of the human epidermal growth factor receptor 2 (HER2) in breast cancer specimens. The software assesses immunohistochemical (IHC) staining reactions of HER2 based on an algorithm evaluating the cell membrane connectivity. The HER2-CONNECTTM algorithm was aligned to match digital image scorings of HER2 performed by 5 experienced assessors in a training set and confirmed in a separate validation set. The training set consisted of 167 breast carcinoma tissue core images in which the assessors individually and blinded outlined regions of interest and gave their HER2 score 0/1+/2+/3+ to the specific tumor region. The validation set consisted of 86 core images where the result of the automated image analysis software was correlated to the scores provided by the 5 assessors. HER2 fluorescence in situ hybridization (FISH) was performed on all cores and used as a reference standard. The overall agreement between the image analysis software and the digital scorings of the 5 assessors was 92.1% (Cohen%26apos;s Kappa: 0.859) in the training set and 92.3% (Cohen%26apos;s Kappa: 0.864) in the validation set. The image analysis sensitivity was 99.2% and specificity 100% when correlated to FISH. In conclusion, the Visiopharm HER2 IHC algorithm HER2-CONNECTTM can discriminate between amplified and non-amplified cases with high accuracy and diminish the equivocal category and thereby provides a promising supplementary diagnostic tool to increase consistency in HER2 assessment.

• 出版日期2012-2