Deletion of flbA Results in Increased Secretome Complexity and Reduced Secretion Heterogeneity in Colonies of Aspergillus niger

作者：Krijgsheld Pauline; Nitsche Benjamin M; Post Harm; Levin Ana M; Muller Wally H; Heck Albert J R; Ram Arthur F J; Altelaar A F Maarten; Wosten Han A B^*

来源：Journal of Proteome Research, 2013, 12(4): 1808-1819.

DOI：10.1021/pr301154w

• fungus
• Aspergillus niger
• heterogeneity
• conidiation
• flbA
• regulator of G protein signaling
• quantitative proteomics
• dimethyl labeling
• secretome
• cell factory

Aspergillus niger is a cell factory for the production of enzymes. This fungus secretes proteins in the central part and at the periphery of the colony. The sporulating zone of the colony overlapped with the nonsecreting subperipheral zone, indicating that sporulation inhibits protein secretion. Indeed, strain Delta flbA that is affected early in the sporulation program secreted proteins throughout the colony. In contrast, the Delta brlA strain that initiates but not completes sporulation did not show altered spatial secretion. The secretome of 5 concentric zones of xylose-grown Delta flbA colonies was assessed by quantitative proteomics. In total 138 proteins with a signal sequence for secretion were identified in the medium of Delta flbA colonies. Of these, 18 proteins had never been reported to be part of the secretome of A. niger, while 101 proteins had previously not been identified in the culture medium of xylose-grown wild type colonies. Taken together, inactivation of flbA results in spatial changes in secretion and in a more complex secretome. The latter may be explained by the fact that strain Delta flbA has a thinner cell wall compared to the wild type, enabling efficient release of proteins. These results are of interest to improve A. niger as a cell factory.

• 出版日期2013-4