科研之友
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摘要
To obtain the initial crystallization hit and the subsequent optimization remain the major bottlenecks in membrane protein structure determination. Here we describe an approach to achieve diffraction quality membrane protein crystals in high concentrations of lipid and detergent (HILIDE). Initial hits are achieved :through a controlled variation of the membrane protein/lipid/detergent ratios, and optimized crystals are established by systematic screening with secondary detergents at concentrations that are adapted for the method. The approach is generally applicable for native and heterologously expressed membrane proteins, and it is compatible with any crystallization method, such as vapor diffusion and batch crystallization under oil. The method provides a user defined lipidic environment and is in itself not limited to lipids with particular properties, such as the lipid-phase crystallization methods.
- 出版日期2011-6
