Deletion of 1.8-kb mRNA of Marek's disease virus decreases its replication ability but not oncogenicity

作者：Sun, Aijun; Li, Yanpeng; Wang, Jingyan; Su, Shuai; Chen, Hongjun; Zhu, Hongfei; Ding, Jiabo^*; Cui, Zhizhong

来源：Virology Journal, 2010, 7: 294.

DOI：10.1186/1743-422X-7-294

• H GENE FAMILY
• RETICULOENDOTHELIOSIS VIRUS
• BIDIRECTIONAL PROMOTER
• PP38
• GENOME
• STRAIN
• TRANSCRIPTS
• PP38/PP24
• INDUCTION
• REPEATS

Background: The 1.8-kb mRNA was reported as one of the oncogenesis-related genes of Marek's disease virus (MDV). In this study, the bacterial artificial chromosome (BAC) clone of a MDV field strain GX0101 was used as the platform to generate mutant MDV to examine the functional roles of 1.8-kb mRNA.
Results: Based on the BAC clone of GX0101, the 1.8-kb mRNA deletion mutant GX0101 Delta(A+C) was constructed. The present experiments indicated that GX0101 Delta(A+C) retained a low level of oncogenicity, and it showed a decreased replication capacity in vitro and in vivo when compared with its parent virus, GX0101. Further studies in vitro demonstrated that deletion of 1.8-kb mRNA significantly decreased the transcriptional activity of the bidirectional promoter between 1.8-kb mRNA and pp38 genes of MDV.
Conclusion: These results suggested that the 1.8-kb mRNA did not directly influence the oncogenesis but related to the replication ability of MDV.

• 出版日期2010-10-29
• 单位中国农业科学院; 山东农业大学; 中国兽医药品监察所; 中国农业科学院上海兽医研究所