Analysis of the miRNA-mRNA networks in malignant transformation BEAS-2B cells induced by alpha-particles

作者：Nie, Ji-Hua; Chen, Zhi-Hai; Shao, Chun-Lin; Pei, Wei-Wei; Zhang, Jie; Zhang, Shu-Yu; Jiao, Yang; Tong, Jian^*

来源：Journal of Toxicology and Environmental Health Part A: Current Issues , 2016, 79(9-10): 427-435.

DOI：10.1080/15287394.2016.1176628

• BRONCHIAL EPITHELIAL-CELLS
• LUNG-CANCER CELLS
• RADON EXPOSURE
• INVASION
• EXPRESSION
• MIGRATION
• PROLIFERATION
• APOPTOSIS
• MIR-107
• GROWTH

The aim of this study was to determine the toxicity induced by irradiation with alpha-particles on malignant transformation of immortalized human bronchial epithelial cells (BEAS-2B) using miRNA-mRNA networks. The expression of BEAS-2B cells was determined by measuring colony formation, mtDNA, mitochondrial membrane potential (MMP), and ROS levels. Changes in BEAS-2B cell gene expression were observed and quantified using microarrays that included an increase in 157 mRNA and 20 miRNA expression and a decrease in 77 mRNA and 48 miRNA. Bioinformatic software was used to analyze these different mRNA and miRNA, which indicated that miR-107 and miR-494 play an important role in alpha-particles-mediated cellular malignant transformation processes. The pathways related to systemic lupus erythematosus, cytokine-cytokine receptor interaction, MAPK signaling pathway, regulation of actin cytoskeleton, and cell adhesion molecules (CAMs) were stimulated, while those of ribosome, transforming growth factor (TGF)-beta signaling pathway, and metabolic pathways were inhibited. Data suggest that miRNA and mRNA play a crucial role in alpha-particles-mediated malignant transformation processes. It is worth noting that three target genes associated with lung cancer were identified and upregulated PEG 10 (paternally expressed gene 10), ARHGAP26, and IRS1.

• 出版日期2016
• 单位苏州大学; 复旦大学