Antitumor Effect of New HER2 Peptide Vaccination Based on B Cell Epitope

作者：Miyako Hirohito; Kametani Yoshie^*; Katano Ikumi; Ito Ryoji; Tsuda Banri; Furukawa Atsuko; Saito Yuki; Ishikawa Dai; Ogino Koichi; Sasaki Shigeru; Imai Kohzoh; Habu Sonoko; Makuuchi Hiroyasu; Tokuda Yutaka

来源：Anticancer Research, 2011, 31(10): 3361-3368.

• Her-2/neu
• peptide
• vaccine
• B-cell epitope
• antitumor effect

Background: While the benefit of passive immunotherapy is commonly accepted, active immunization may have advantages for the patient's quality of life. We identified a new epitope of Mab CH401 against Her-2/neu extracellular domain (N: 167-175), and evaluated the effect of active immunization of the 20mer peptide containing the epitope (CH401 peptide). Materials and Methods: Epitope-mapping was performed using ELISA with Her-2/neu-related multiple antigen peptides (MAP). BALB/c mice were transplanted with Her-2/neu-expressing lymphoma cell line and immunized with the peptides. For monitoring the condition, ELISA and flow cytomeny was performed. Results: CH401 peptide induced Her-2/neu-specific IgG antibody. Tumor growth in immunized mice was suppressed and tumor-infiltrating lymphocytes comprised snore CD8(+) T-cells, which secreted larger amounts of interleukin-2 after the peptide re-stimulation. Conclusion: The new Her-2/neu peptide contained epitopes for CD4(+) and CD8(+) T-cells, which contributes to the suppressive effect on Her-2/neu-expressing tumor cell growth.

• 出版日期2011-10