Anti-complementary activity of two homogeneous polysaccharides from Eclipta prostrata

作者：Wang, Hongwei; Li, Ning; Zhu, Chao; Shi, Songshan; Jin, Hong; Wang, Shunchun^*

来源：Biochemical and Biophysical Research Communications, 2017, 493(2): 887-893.

DOI：10.1016/j.bbrc.2017.09.126

• Eclipta prostrata
• Polysaccharide
• Isolation
• Anticomplement activity

Complement-mediated inflammation and tissue damage is an important drive to pathology in autoimmune diseases, targeting inhibit the complement activation is promising treatment strategy for these diseases. We performed anticomplement activity-guided fractionation of the water extract of Eclipta prostrata by ion-exchange and size-exclusion chromatography, yielding two bioactive polysaccharides EAP20-1 and EAP20-2. The molecular weights of EAP20-1 and EAP20-2 were respectively calculated to be 5.2 kDa and 6.3 kDa by HPGPC, both polysaccharides was composed by D-Gal, L-Glc, and Ara at different ratios of 7.3:2.7:1 and 7.6:3.1:1. In addition, the main linkage types of EAP20-1 and EAP20-2 were beta-1,4-Gal, beta-1,6-Gal and alpha-1,4,6-Glc according to methylation analyses. EAP20-1 and EAP20-2 exhibited significant inhibitory effect on the complement activation through both classical and alternative pathways and with no influence on the coagulation system. Preliminary mechanism study indicated that both EAP20-1 and EAP20-2 inhibited the activation of the complement system by interacting with C1q, C1r, C1s, C2, C4, C5, C7, and C9 components.

• 出版日期2017-11-18
• 单位上海中医药大学