DETERMINATION OF CHROMOSOME SIZE AND NUMBER OF RRN LOCI IN LACTOBACILLUS-PLANTARUM BY PULSED-FIELD GEL-ELECTROPHORESIS

作者：CHEVALLIER B; HUBERT JC; KAMMERER B

来源：FEMS Microbiology Letters, 1994, 120(1-2): 51-56.

• LACTOBACILLUS PLANTARUM
• LACTOBACILLUS PENTOSUS
• PULSED-FIELD GEL ELECTOPHORESIS
• RM LOCI
• GENOME SIZE
• RESTRICTION FRAGMENT LENGTH POLYMORPHISM

The size of the Lactobacillus plantarum CCM 1904 chromosome was determinated by pulse-field gel electrophoresis. It was found to be 3.3-3.4 Mb using SfiI or AscI restriction endonucleases, compared to 3-4 Mb found for the other L. plantarum strains tested. L. plantarum CCM 1904 5S rDNA was cloned by polymerase chain reaction, sequenced, and used as a probe to characterize strains. At least five rrn loci were found. The pulsed-field gel electrophoresis macrorestriction patterns were strain-specific, while the rDNA restriction hybridization patterns were species-specific.

• 出版日期1994-7-1