Application of 3 mu m particle-based amylose-derived chiral stationary phases for the enantioseparation of potential histone deacetylase inhibitors

作者：Ferretti Rosella; Mai Antonello; Gallinella Bruno; Zanitti Leo; Valente Sergio; Cirilli Roberto^*

来源：Journal of Chromatography A, 2011, 1218(46): 8394-8398.

DOI：10.1016/j.chroma.2011.09.052

• Enantiomer separation
• Chiralpak IA-3
• Chiralpak AD-3
• Temperature effects
• Conformational transition
• Absolute configuration

In this work, we report on the difference in performance of the two 3 m particle-based Chiralpak IA-3 and Chiralpak AD-3 chiral stationary phases (CSPs) in the direct resolution of four racemic cinnamyl 2-aminoanilides, endowed with histone deacetylase inhibitory activity. The 3 mu m CSPs were explored to determine if they could provide an effective resolution of enantiomers in presence of alcoholic eluents such as pure methanol, ethanol and 2-propanol. Temperature variable enantioselective HPLC and subsequent van't Hoff analysis were performed. In most of cases the van't Hoff plots were found to show a non-linear behaviour. The knowledge of the enantiomeric elution order associated with the data coming from enantioselective HPLC permitted to advance some hypothesis about the groups involved in chiral recognition mechanism.

• 出版日期2011-11-18