A novel glass ionomer cement containing MgCO3 apatite induced the increased proliferation and differentiation of human pulp cells in vitro

作者：Laiteerapong Arunee; Lochaiwatana Yossakit; Hirata Isao; Okazaki Masayuki; Mori Kenta; Murakami Shinya; Poolthong Suchit^*

来源：Dental Materials Journal, 2012, 31(5): 772-778.

DOI：10.4012/dmj.2012-096

• Glass ionomer
• Magnesium carbonate apatite
• Biological response
• Pulp-dentin regeneration

This study aimed to investigate the in vitro biological response of human dental pulp cells to glass ionomer cement (GIC, Fuji IX GP (R)) containing 2.5% magnesium carbonate apatite (MgCO(3)Ap). MgCO(3)Ap was synthesized by wet method and characterized using FT-IR, XPS, and SEM. Fuji IX GP (R) served as a control. Test and control cements were prepared by encapsulated mixing the powder with Fuji IX-liquid (P/L=3.6:1). Eluates from cements extracted by 1 mL culture medium were collected at day 1, 7 and 14, and used for WST-1 proliferation assay. For ALPase activity, cells were maintained with cements in transwells, harvested and enzyme activity was measured at day 1, 4, 7, 14, and 21. We found a higher cell proliferation and increased ALPase activity by pulp cells in the test group compared to the control. This suggests the potential of GIC containing this novel biological apatite as a restorative material for pulp-dentin regeneration.

• 出版日期2012-9