A new strategy for site-specific alkylation of DNA using oligonucleotides containing an abasic site and alkylating probes

作者：Sato Norihiro; Tsuji Genichiro; Sasaki Yoshihiro; Usami Akira; Moki Takuma; Onizuka Kazumitsu; Yamada Ken; Nagatsugi Fumi^*

来源：Chemical communications, 2015, 51(80): 14885-14888.

DOI：10.1039/c5cc03915k

Selective chemical reactions with DNA, such as its labelling, are very useful in many applications. In this paper, we discuss a new strategy for the selective alkylation of DNA using an oligonucleotide containing an abasic site and alkylating probes. We designed three probes consisting of 2-AVP as a reactive moiety and three kinds of binding moiety with high affinity to duplex DNA. Among these probes, Hoechst-AVP probe exhibited high selectivity and efficient reactivity to thymine bases at the site opposite an abasic site in DNA. Our method is potentially useful for inducing site-directed reactions aimed at inhibiting polymerase reactions.

• 出版日期2015