An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+T cell responses in a mouse model

作者：Chahal Jasdave S; Fang Tao; Woodham Andrew W; Khan Omar F; Ling Jingjing; Anderson Daniel G; Ploegh Hidde L^*

来源：Scientific Reports, 2017, 7(1): 252.

DOI：10.1038/s41598-017-00193-w

The Zika virus (ZIKV) outbreak in the Americas and South Pacific poses a significant burden on human health because of ZIKV's neurotropic effects in the course of fetal development. Vaccine candidates against ZIKV are coming online, but immunological tools to study anti-ZIKV responses in preclinical models, particularly T cell responses, remain sparse. We deployed RNA nanoparticle technology to create a vaccine candidate that elicited ZIKV E protein-specific IgG responses in C57BL/ 6 mice as assayed by ELISA. Using this tool, we identified a unique H-2Db-restricted epitope to which there was a CD8+ T cell response in mice immunized with our modified dendrimer-based RNA nanoparticle vaccine. These results demonstrate that this approach can be used to evaluate new candidate antigens and identify immune correlates without the use of live virus.

• 出版日期2017-3-21