aPKC lambda controls epidermal homeostasis and stem cell fate through regulation of division orientation

作者：Niessen Michaela T; Scott Jeanie; Zielinski Julia G; Vorhagen Susanne; Sotiropoulou Panagiota A; Blanpain Cedric; Leitges Michael^*; Niessen Carien M

来源：The Journal of Cell Biology, 2013, 202(6): 887-900.

DOI：10.1083/jcb.201307001

The atypical protein kinase C (aPKC) is a key regulator of polarity and cell fate in lower organisms. However, whether mammalian aPKCs control stem cells and fate in vivo is not known. Here we show that loss of aPKC lambda in a self-renewing epithelium, the epidermis, disturbed tissue homeostasis, differentiation, and stem cell dynamics, causing progressive changes in this tissue. This was accompanied by a gradual loss of quiescent hair follicle bulge stem cells and a temporary increase in proliferating progenitors. Lineage tracing analysis showed that loss of aPKC lambda altered the fate of lower bulge/hair germ stem cells. This ultimately led to loss of proliferative potential, stem cell exhaustion, alopecia, and premature aging. Inactivation of aPKC lambda produced more asymmetric divisions in different compartments, including the bulge. Thus, aPKC lambda is crucial for homeostasis of self-renewing stratifying epithelia, and for the regulation of cell fate, differentiation, and maintenance of epidermal bulge stem cells likely through its role in balancing symmetric and asymmetric division.

• 出版日期2013-9-16