A software tool to accelerate design of protein constructs for recombinant expression

作者：Sagemark Johanna; Kraulis Per; Weigelt Johan^*

来源：Protein Expression and Purification, 2010, 72(2): 175-178.

DOI：10.1016/j.pep.2010.03.020

• Sequence analysis
• Structure prediction
• Construct design
• Protein expression
• Protein crystallization

Structural and biochemical analysis of proteins requires access to purified protein material. Modern molecular biology technologies facilitate straightforward molecular cloning and expression analysis of multiple protein constructs in parallel, and such approaches have proven very efficient to identify samples suitable for further analysis.
A variety of information can be used to support rational design of protein constructs. This includes, e.g. prediction of secondary structure elements, protein domain predictions, and structure prediction methods such as threading. To fully access the available information, collation of data extracted from several different sources is required. This can be cumbersome and sometimes also confusing due to for example different implementation of amino acid residue numbering schemes. The SGC Domain Boundary Analyser tool provides a graphical interface that simplifies and accelerates rational design of protein expression constructs.

• 出版日期2010-8