Methylation analysis of idiopathic recurrent spontaneous miscarriage cases reveals aberrant imprinting at H19 ICR in normozoospermic individuals

作者：Ankolkar Mandar; Patil Anushree; Warke Himangi; Salvi Vinita; Mokashi Neelam Kedia; Pathak Shilpa; Balasinor N H^*

来源：Fertility and Sterility, 2012, 98(5): 1186-1192.

DOI：10.1016/j.fertnstert.2012.07.1143

• Recurrent spontaneous miscarriage
• H19 ICR
• DNA methylation
• CMA3 analysis

Objective: To study H19 ICR methylation levels in association with sperm parameters routinely analyzed in idiopathic recurrent spontaneous miscarriage cases.
Design: Case-control study.
Setting: Academic research setting.
Patient(s): Male partners of couples with a history of idiopathic recurrent spontaneous miscarriage (RSM group) and male partners of couples with proven fertility (control group).
Intervention(s): None.
Main Outcome Measure(s): Paternal age, sperm concentration, motility, chromatin compaction status, morphology, and H19 ICR methylation were assessed in control and idiopathic RSM group participants.
Result(s): Paternal age and basic semen parameters analyzed did not show any significant difference between the two groups; however H19 ICR methylation levels were reduced significantly in the idiopathic RSM group compared with the control group.
Conclusion(s): Significant reduction in the H19 ICR methylation without significant difference in the sperm parameters demonstrates aberrant imprinting to be associated with idiopathic RSM. (Fertil Steril (R) 2012;98:1186-92.

• 出版日期2012-11