Nanopore Diameters Tune Strain in Extruded Fibronectin Fibers

作者：Raoufi Mohammad; Das Tamal; Schoen Ingmar; Vogel Viola; Brueggemann Dorothea; Spatz Joachim P

来源：Nano Letters, 2015, 15(10): 6357-6364.

DOI：10.1021/acs.nanolett.5b01356

• conformational changes
• protein nanofiber
• AAO template
• FRET
• extrusion

Fibronectin is present in the extracellular matrix and can be assembled into nanofibers in vivo by undergoing conformational changes. Here, we present a novel approach to prepare fibronectin nanofibers under physiological conditions using an extrusion approach through nanoporous aluminum oxide membranes. This one-step process can prepare nanofiber bundles up to a millimeter in length and with uniform fiber diameters in the nanometer range. Most importantly, by using different pore diameters and protein concentrations in the extrusion process, we could induce varying lasting structural changes in the fibers, which were monitored by Forster resonance energy transfer and should impose different physiological functions.

• 出版日期2015-10