Molecular Cloning and Characterization of Carotenoid Pathway Genes and Carotenoid Content in Ixeris dentata var. albiflora

作者：Reddy Chinreddy Subramanyam; Lee Sang Hoon; Yoon Jeong Su; Kim Jae Kwang; Lee Sang Won; Hur Mok; Koo Sung Cheol; Kim Mi Ran; Lee Woo Moon; Jang Jae Ki; Hur Yoonkang; Park Sang Un; Kim Yeon Bok

来源：Molecules, 2017, 22(9): 1449.

DOI：10.3390/molecules22091449

• Ixeris dentata var. albiflora
• carotenoid
• transcript level

Ixeris dentata var. albiflora is considered as a potential therapeutic agent against mithridatism, calculous, indigestion, pneumonia, hepatitis, and tumors as well as good seasoned vegetable in Far East countries. Phytoene synthase (PSY), phytoene desaturase (PDS) xi-carotene desaturase (ZDS), lycopene beta-cyclase (LCYB), lycopene epsilon-cyclase (LCYE), epsilon-ring carotene hydroxylase (CHXB), and zeaxanthin epoxidase (ZDS) are vital enzymes in the carotenoid biosynthesis pathway. We have examined these seven genes from I. dentata that are participated in carotenoid biosynthesis utilizing an Illumina/Solexa HiSeq 2000 platform. In silico analysis of the seven deduced amino acid sequences were revealed its closest homology with other Asteracea plants. Further, we explored transcript levels and carotenoid accumulation in various organs of I. dentata using quantitative real time PCR and high-performance liquid chromatography, respectively. The highest transcript levels were noticed in the leaf for all the genes while minimal levels were noticed in the root. The maximal carotenoid accumulation was also detected in the leaf. We proposed that these genes expressions are associated with the accumulation of carotenoids. Our findings may suggest the fundamental clues to unravel the molecular insights of carotenoid biosynthesis in various organs of I. dentata.

• 出版日期2017-9