Detection of GSK-3β activation index in pediatric chronic tonsillitis is an indicator for chronic recurrent inflammation

作者：Gao, Yunfei; Mi, Jiaoping; Chen, Fenghong; Liao, Zhenpeng; Feng, Xiaoshan; Lv, Minghui; He, Haixin; Cao, Yujie; Yan, Yan; Zhu, Zhe; Fan, Yunping^*; Hong, Haiyu^*

来源：American Journal of Otolaryngology, 2018, 39(3): 277-281.

DOI：10.1016/j.amjoto.2018.03.005

• Tonsillitis
• GSK-3
• NF-kappa B
• Phosphorylation
• Palatine tonsil

Purpose: Chronic tonsillitis (TC) is among the most common bacterial diseases in pediatric otolaryngology. We aimed to evaluate the expression of glycogen synthase kinase 3 beta (GSK-3 beta) in a cohort of children with chronic tonsillitis (TC), and the correlation between GSK-3 beta activity index and inflammatory profiles of TC. @@@ Materials and methods: The expression of GSK-3 beta was comparably evaluated between children with TC (n = 26) and tonsillar hypertrophy (TH, n = 26). GSK-3 beta expression was detected by immunohistochemistry, RT-qPCR, and Western blot. The inflammatory profiles between the TC and TH groups were also evaluated. @@@ Results: We found that while GSK-3 beta was highly expressed in both TC and TH groups, no significant difference were detected at mRNA and protein levels between groups. The protein level of p-GSK-3 beta was significantly lower in the TC group as compared to the TH group. Additionally, the inflammatory markers, including NF-kappa B, T-bet, and IFN-gamma were higher in the TC group compared to TH group. The GSK-3 beta activation index was positively correlated with the levels of NF-kappa B, T-bet, and IFN-gamma in the TC group. @@@ Conclusions: Our findings suggested that GSK-3 beta activation index was demonstrated to be a clinically applicable indicator for chronic recurrent inflammation in pediatric TC.

• 出版日期2018-5
• 单位中山大学