An alternative beads-on-a-string chromatin architecture in Thermococcus kodakarensis

作者：Maruyama Hugo; Harwood Janet C; Moore Karen M; Paszkiewicz Konrad; Durley Samuel C; Fukushima Hisanori; Atomi Haruyuki; Takeyasu Kunio; Kent Nicholas A^*

来源：EMBO Reports, 2013, 14(8): 711-717.

DOI：10.1038/embor.2013.94

• chromatin
• nucleosome
• histone
• archaea
• chromatin-seq

We have applied chromatin sequencing technology to the euryarchaeon Thermococcus kodakarensis, which is known to possess histone-like proteins. We detect positioned chromatin particles of variable sizes associated with lengths of DNA differing as multiples of 30 bp (ranging from 30 bp to 4450 bp) consistent with formation from dynamic polymers of the archaeal histone dimer. T. kodakarensis chromatin particles have distinctive underlying DNA sequence suggesting a genomic particle-positioning code and are excluded from gene-regulatory DNA suggesting a functional organization. Beads-on-a-string chromatin is therefore conserved between eukaryotes and archaea but can derive from deployment of histone-fold proteins in a variety of multimeric forms.

• 出版日期2013-8