MicroRNA-497 Suppresses Proliferation and Induces Apoptosis in Prostate Cancer Cells

作者：Wang, Li^*; Li, Bo; Li, Lei; Wang, Te

来源：Asian Pacific Journal of Cancer Prevention, 2013, 14(6): 3499-3502.

DOI：10.7314/APJCP.2013.14.6.3499

• MiR-497
• prostate cancer
• apoptosis
• growth reduction

MicroRNAs (miRNAs) are a class of endogenously expressed small, non-coding, single-stranded RNAs that negatively regulate gene expression, mainly by binding to 3'-untranslated regions (3'UTR) of their target messenger RNAs (mRNAs), which cause blocks of translation and/or mRNA cleavage. Recently, miRNAprofiling studies demonstrated the microRNA-497 (miR-497) level to be down-regulated in all prostate carcinomas compared with BPH samples. The purpose of this study was to investigate the potential role of miR-497 in human prostate cancer. Proliferation, cell cycle and apoptosis assays were conducted to explore the potential function of miR-497 in human prostate cancer cells. Results showed that miR-497 suppressed cellular growth and initiated G0/G1 phase arrest of LNCaP and PC-3 cells. We also observed that miR-497 increased the percentage of apoptotic cells by increasing caspase-3/7 activity. Taken together, our results demonstrated that miR-497 can inhibit growth and induce apoptosis by caspase-3 activation in prostate cancer cells, which suggest its use as a potential therapeutic target in the future.

• 出版日期2013
• 单位北京师范大学