摘要

This study aimed to determine the optimal dietary niacin requirement of juvenile Megalobrama amblycephala. Fish, with an average weight of 3.62 +/- 0.02g, were randomly divided into six groups and fed six purified diets with graded levels of niacin (0, 10, 20, 30, 40 and 50mg/kg) three times daily for 8weeks. The survival rate, weight gain (WG), feed conversion ratio, protein efficiency and protein retention all significantly improved (p<.05) as the dietary niacin level increased from 0 to 30mg/kg but showed no significant difference (p>.05) with further increases in niacin levels. The supplementation of 30mg/kg niacin also led to a significant (p<.01) reduction in the viscerosomatic index, hepatosomatic index and mesenteric fat index. The dietary niacin levels exhibited minimal effects on whole-body moisture and ash content (p>.05) but exerted a significant (p<.05) influence on protein and lipid contents and liver nicotinamide concentrations with high values obtained in fish receiving greater than 30mg/kg of niacin. In addition, moderate levels of niacin lowered plasma triglycerides, non-esterified fatty acid and low-density lipoprotein cholesterol concentrations (p<.05). Based on the broken-line regression analysis of WG and liver nicotinamide content, the optimal dietary niacin requirement of juvenile blunt snout bream was 31.25 and 30.62mg/kg, respectively.