科研之友
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摘要
Grain proteins from 173 rice landraces (Oryza sativa L.) were electrophoretically separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Densitometric scanning of the electrophoretic profiles permitted the estimation of the relative concentration of 25 polypeptide fractions, which were used as variables for the calculation of similarity coefficients between these accessions. A considerable variation was observed in glutelin subunits and Wx gene product. Very low variation was observed in prolamin subunits. Lower genetic distance based on total seed protein profile may be attributed to narrow genetic base. This electrophoretically detectable protein polymorphism in rice grain can be used in further breeding proposes and variety development. Our results suggest that screening of landraces on the basis of variation in seed storage protein profile using SDS-PAGE could be highly effective tool to identify valuable rice genetic resources.
- 出版日期2011-6
