Development of new UV-vis spectroscopic microwave-assisted method for determination of glucose in pharmaceutical samples

作者：Mabood Fazal^*; Hussain Z; Haq H; Arian M B; Boque R; Khan K M; Hussain K; Jabeen F; Hussain J; Ahmed M; Alharasi A; Naureen Z; Hussain H; Khan A; Perveen S

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2016, 153: 212-215.

DOI：10.1016/j.saa.2015.08.035

• Glucose analysis
• Microwave synthesizer
• UV-vis spectrophotometry
• Carbohydrates
• Aniline
• Molybdenum blue complex
• Microwave assisted synthesis

A new UV-Visible spectroscopic method assisted with microwave for the determination of glucose in pharmaceutical formulations was developed. In this study glucose solutions were oxidized by ammonium molybdate in the presence of microwave energy and reacted with aniline to produce a colored solution. Optimum conditions of the reaction including wavelength, temperature, and pH of the medium and relative concentration ratio of the reactants were investigated. It was found that the optimal wavelength for the reaction is 610 nm, the optimal reaction time is 80 s, the optimal reaction temperature is 160 degrees C, the optimal reaction pH is 4, and the optimal concentration ratio aniline/ammonium molybdate solution was found to be 1:1. The limits of detection and quantification of the method are 0.82 and 2.75 ppm for glucose solution, respectively. The use of microwaves improved the speed of the method while the use of aniline improved the sensitivity of the method by shifting the wavelength.

• 出版日期2016-1-15