AFM imaging of RGD presenting synthetic extracellular matrix using gold nanoparticles

作者：Hsiong Susan X; Cooke Peter H; Kong Hyun Joon; Fishman Marshall L; Ericsson Maria; Mooney David J^*

来源：Macromolecular Bioscience, 2008, 8(6): 469-477.

DOI：10.1002/mabi.200700313

• atomic force microscopy (AFM)
• biomaterials
• electron microscopy
• hydrogels
• TEM

Several high-resolution imaging techniques such as FESEM, TEM and AFM are compared with respect to their application on alginate hydrogels, a widely used polysaccharide biomaterial. A new AFM method applicable to RGD peptides covalently conjugated to alginate hydrogels is described. High-resolution images of RGD adhesion ligand distribution were obtained by labeling biotinylated RGD peptides with streptavidin-labeled gold nanoparticles. This method may broadly provide a useful tool for sECM characterization and design for tissue regeneration strategies.

• 出版日期2008-6-11