Development of consensus qPCR primers to detect cytokine genes in three amberjack species: Seriola quinqueradiata, S-lalandi and S-dumerili

作者：Jirapongpairoj Walissara; Kobayashi Keigo; Fukuda Yutaka; Takano Tomokazu; Sakai Takamitsu; Matsuyama Tomomasa; Nakayasu Chihaya; Nozaki Reiko; Hirono Ikuo; Kondo Hidehiro^*

来源：Fisheries Science, 2015, 81(5): 907-914.

DOI：10.1007/s12562-015-0913-4

• Cytokine
• Amberjacks
• Consensus primer
• qPCR

Partial sequences of cytokine genes IFN gamma, IL-1 beta, IL-8, IL-10, and TNF alpha were identified from next-generation sequencing data for three amberjack species: Seriola quinqueradiata, S. lalandi and S. dumerili. The cytokine genes had more than 90 % nucleotide identity among the three species. Quantitative PCR (qPCR) primers were designed from the consensus sequences to detect cytokine genes. The specificities of consensus qPCR primers were investigated in the head kidney of three amberjack species treated with formalin-killed cells (FKC) of Vibrio anguillarum and polyinosinic-polycytidylic acid [poly(I:C)] at 3 h post injection (hpi) using qPCR. The primers detected up-regulation of certain cytokine genes after FKC of V. anguillarum and poly(I:C) stimulation in all species examined. Consequently, the primers are suitable for studying and detecting cytokine genes after vaccination and pathogen infection in these three amberjack species.

• 出版日期2015-9