An electrochemical immunosensor for simultaneous point-of-care cancer markers based on the host-guest inclusion of beta-cyclodextrin-graphene oxide

作者：Feng, Taotao; Qiao, Xiuwen^*; Wang, Haining; Sun, Zhao; Qi, Yu^*; Hong, Chenglin^*

来源：Journal of Materials Chemistry B, 2016, 4(5): 990-996.

DOI：10.1039/c5tb02480c

• GOLD NANOPARTICLES
• NANOCOMPOSITES

A novel electrochemical immunosensor was developed for the simultaneous detection of alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA) using Cu2O-graphene oxide-beta-cyclodextrin (Cu2O-GO-CD) and beta-cyclodextrin-graphene oxide-ferrocenecarboxylic acid (GO-CD-Fc-COOH) as the distinguishable signal probe, and graphene oxide-gold nanoparticles (GO-AuNPs) as the sensor platform. GO-CD displayed excellent solubility in water and rich capture capability to Fc-COOH and the secondary antibody. The proposed immunosensor exhibited an excellent electrochemical performance. The linear ranges were from 0.001 ng mL(-1) to 80 ng mL(-1) for AFP and CEA with a detection limit of 0.0002 ng mL(-1) for AFP and 0.0001 ng mL(-1) for CEA. With the merits of acceptable stability, high sensitivity, a wide linear range and a low detection limit, the proposed immunosensor showed great potential for the simultaneous detection of multi-analytes in clinical diagnosis.

• 出版日期2016
• 单位石河子大学