Nucleotide sequence of the uricase gene from Bacillus sp TB-90

作者：Yamamoto K^*; Kojima Y; Kikuchi T; Shigyo T; Sugihara K; Takashio M; Emi S

来源：Journal of Biochemistry, 1996, 119(1): 80-84.

DOI：10.1093/oxfordjournals.jbchem.a021219

• Bacillus sp
• nucleotide sequence
• processing C-terminus
• thermostability
• uricase

The nucleotide sequence of the uricase gene from the thermophilic bacterium Bacillus sp, TB-90 was determined. The primary structure of the uricase deduced from the nucleotide sequence comprised 332 amino acids, with a total molecular mass of 37,994 Da. The molecular mass of the subunit of the uricase produced by the transformant of Escherichia coli agreed well with this value. However, the molecular mass of a subunit of the uricase produced by Bacillus sp. TB-90 was found to be 34,000 Da by SDS-PAGE. The difference between these molecular masses was attributed to processing of the C-terminal 13 amino acid residue in Bacillus sp. TB-90. Comparison of the enzymatic properties of both uricases showed that the thermostability of the uricase produced by the transformant was enhanced by about 10 degrees C in comparison to that produced by Bacillus sp. TB-90.

• 出版日期1996-1