Motivated Behaviors and Levels of 3,5-THP in the Midbrain Are Attenuated by Knocking Down Expression of Pregnane Xenobiotic Receptor in the Midbrain Ventral Tegmental Area of Proestrous Rats

作者：Frye Cheryl Anne; Koonce Carolyn J; Walf Alicia A; Rusconi Jamie C

来源：The Journal of Sexual Medicine, 2013, 10(7): 1692-1706.

DOI：10.1111/jsm.12173

• Allopregnanolone
• Lordosis
• Progesterone
• Neurosteroids
• Mating
• Receptivity

Introduction Progesterone (P-4) and its product, 5-pregnan-3-ol-20-one (3,5-THP), act in the midbrain ventral tegmental area (VTA) to alter motivated behaviors, such as mating, and motor and anxiety behavior. Of interest is whether 3,5-THP formation requires the pregnane xenobiotic receptor (PXR), which is expressed in the midbrain of rats. %26lt;br%26gt;Aim The role of PXR in the midbrain for 3,5-THP formation, which precedes modulation of motivated behaviors, was investigated. %26lt;br%26gt;Methods Rats had estrous cycle phase determined and were assessed when they were in diestrus or proestrus. Diestrous and proestrous rats were infused with control or antisense oligodeoxyribonucleotides (AS-ODNs) targeted against PXR to the VTA. %26lt;br%26gt;Main Outcome Measures In pilot studies, PXR gene and protein expression in the midbrain were determined with quantitative reverse transcriptase polymerase chain reaction and Western blotting, respectively. Diestrous and proestrous rats infused with control or AS-ODNs to the VTA were tested for anxiety (open field and plus maze), social (social interaction), and sexual (paced mating) behavior. Expression of PXR in the midbrain was verified with Western blotting. Plasma estradiol, P-4, dihydroprogesterone (DHP), and 3,5-THP levels, and brain P-4, DHP, and 3,5-THP levels were measured. We predicted that proestrous rats infused with PXR AS-ODNs would have decreased anti-anxiety, social, and sexual behavior, lower midbrain expression of PXR, and lower midbrain levels of 3,5-THP compared with controls. %26lt;br%26gt;Results Results supported the hypothesis that formation of 3,5-THP requires PXR and may be important for motivated behaviors. PXR AS-ODN, compared with control, infusions to the VTA reduced PXR expression and 3,5-THP levels in the midbrain and attenuated sexual receptivity of proestrous rats. %26lt;br%26gt;Conclusions Knockdown of PXR in the midbrain reduces 3,5-THP levels and sexual receptivity of proestrous rats. Thus, PXR in the midbrain may be required for the observed increase in 3-5-THP during proestrus, which has subsequent effects on motivated, reproductive behaviors. Frye CA, Koonce CJ, Walf AA, and Rusconi JC. Motivated behaviors and levels of 3,5-THP in the midbrain are attenuated by knocking down expression of pregnane xenobiotic receptor in the midbrain ventral tegmental area of proestrous rats. J Sex Med 2013;10:1692-1706.

• 出版日期2013-7