alpha-Cyperone, isolated from the rhizomes of Cyperus rotundus, inhibits LPS-induced COX-2 expression and PGE(2) production through the negative regulation of NF kappa B signalling in RAW 264.7 cells

作者：Jung Seung Hyun; Kim Su Jung; Jun Bo Gyu; Lee Kyung Tae; Hong Seon Pyo; Oh Myung Sook; Jang Dae Sik^*; Choi Jung Hye

来源：Journal of Ethnopharmacology, 2013, 147(1): 208-214.

DOI：10.1016/j.jep.2013.02.034

• alpha-cyperone
• Rhizomes of Cyperus rotundus
• Inflammation
• NF kappa B
• COX-2
• PGE(2)

Ethnopharmacological relevance: The rhizomes of Cyperus rotundus (Cyperaceae) have been used in Asian traditional medicine for the treatment of several inflammatory diseases. However, the anti-inflammatory effects of alpha-cyperone, a major active compound of Cyperus rotundus, are poorly understood. %26lt;br%26gt;Materials and methods: PGE(2) and cytokines released from cells were measured using an EIA assay kit. The expression of iNOS, COX-2, TNF-alpha, and IL-6 was measured by real-time RT-PCR and/or Western blot analysis. A luciferase assay was performed to measure the effect of alpha-cyperone on NF kappa B activity. %26lt;br%26gt;Results: The n-hexane fraction of the 80% EtOH extract from the rhizomes of Cyperus rotundus was found to inhibit both NO and PGE(2) production in RAW 264.7 cells. alpha-Cyperone isolated from the n-hexane fraction significantly inhibited PGE(2) production by suppressing the LPS-induced expression of inducible COX-2 at both the mRNA and the protein levels. In contrast, alpha-cyperone had little effect on NO production and iNOS expression. Additionally, alpha-cyperone downregulated the production and mRNA expression of the inflammatory cytokine IL-6. Moreover, treatment with alpha-cyperone suppressed the transcriptional activity of NF kappa B and the nuclear translocation of the p65 NF kappa B subunit in LPS-induced RAW 264.7 cells. %26lt;br%26gt;Conclusion: The anti-inflammatory activity of alpha-cyperone is associated with the down-regulation of COX-2 and IL-6 via the negative regulation of the NF kappa B pathway in LPS-stimulated RAW 264.7 cells.

• 出版日期2013-5-2