Ultrasensitive Detection of Human Liver Hepatocellular Carcinoma Cells Using a Label-Free Aptasensor

作者：Kashefi Kheyrabadi Leila; Mehrgardi Masoud A^*; Wiechec Emilia; Turner Anthony P F; Tiwari Ashutosh

来源：Analytical Chemistry, 2014, 86(10): 4956-4960.

DOI：10.1021/ac500375p

Liver cancer is one of the most common cancers in the world and has no effective cure, especially in later stages. The development of a tangible protocol for early diagnosis of this disease remains a major challenge. In the present manuscript, an aptamer-based, label-free electrochemical biosensor for the sensitive detection of HepG2, a hepatocellular carcinoma cell line, is described. The target cells are captured in a sandwich architecture using TLS11a aptamer covalently attached to a gold surface and a secondary TLS11a aptamer. The application of TLS11a aptamer as a recognition layer resulted in a sensor with high affinity for HepG2 cancer cells in comparison with control cancer cells of human prostate, breast, and colon tumors. The aptasensor delivered a wide linear dynamic range over 1 X 10(2) to 1 X 10(6) cells/mL, with a detection limit of 2 cells/mL. This protocol provides a precise method for sensitive detection of liver cancer with significant advantages in terms of simplicity, low cost, and stability.

• 出版日期2014-5-20