Flavonol-rich fractions of yaupon holly leaves (Ilex vomitoria, Aquifoliaceae) induce microRNA-146a and have anti-inflammatory and chemopreventive effects in intestinal myofribroblast CCD-18Co cells

作者：Noratto Giuliana D; Kim Youngmok; Talcott Stephen T; Mertens Talcott Susanne U^*

来源：Fitoterapia, 2011, 82(4): 557-569.

DOI：10.1016/j.fitote.2011.01.013

• CCD-18Co colon cells
• Yaupon holly (Ilex vomitoria, Aquifoliaceae)
• Colon inflammation
• MicroRNA-146a

Polyphenolics extracted from yaupon holly (Ilex vomitoria, Aquifoliaceae) (YH) leaves were investigated in human colon cells for their chemopreventive and anti-inflammatory activities. An activity-guided fractionation allowed the selection of YH flavonol-rich fraction due to its preferential inhibition of HT-29 colon cancer viability over the normal CCD-18Co colon cells. Quercetin and kaempferol 3-rutinosides, main components identified in this fraction, protected CCD-18Co cells against reactive oxidative species (ROS) in part due to increased activity of antioxidant enzymes. In addition, up-regulation of microRNA-146a (miR-146a) known as a negative regulator of pro-inflammatory NF-kappa B activation was the underlying molecular mechanism that protected CCD-18Co from inflammation.

• 出版日期2011-6