Alginate-Derived Oligosaccharide Inhibits Neuroinflammation and Promotes Microglial Phagocytosis of beta-Amyloid

作者：Zhou, Rui; Shi, Xu Yang; Bi, De Cheng; Fang, Wei Shan; Wei, Gao Bin; Xu, Xu^*

来源：Marine Drugs, 2015, 13(9): 5828-5846.

DOI：10.3390/md13095828

• alginate
• -amyloid
• microglia
• neuroinflammation
• phagocytosis
• toll-like receptor 4

Alginate from marine brown algae has been widely applied in biotechnology. In this work, the effects of alginate-derived oligosaccharide (AdO) on lipopolysaccharide (LPS)/-amyloid (A)-induced neuroinflammation and microglial phagocytosis of A were studied. We found that pretreatment of BV2 microglia with AdO prior to LPS/A stimulation led to a significant inhibition of production of nitric oxide (NO) and prostaglandin E-2 (PGE(2)), expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and secretion of proinflammatory cytokines. We further demonstrated that AdO remarkably attenuated the LPS-activated overexpression of toll-like receptor 4 (TLR4) and nuclear factor (NF)-B in BV2 cells. In addition to the impressive inhibitory effect on neuroinflammation, we also found that AdO promoted the phagocytosis of A through its interaction with TLR4 in microglia. Our results suggested that AdO exerted the inhibitory effect on neuroinflammation and the promotion effect on microglial phagocytosis, indicating its potential as a nutraceutical or therapeutic agent for neurodegenerative diseases, particularly Alzheimer's disease (AD).

• 出版日期2015-9
• 单位深圳大学