Antiproliferative effects of anastrozole, methotrexate, and 5-fluorouracil on endometriosis in vitro and in vivo

作者：Ngo Charlotte; Nicco Carole; Leconte Mahaut; Chereau Christiane; Weill Bernard; Batteux Frederic^*; Chapron Charles

来源：Fertility and Sterility, 2010, 94(5): 1632-U464.

DOI：10.1016/j.fertnstert.2009.09.031

• Endometriosis
• anastrozole
• methotrexate
• 5 fluorouracil
• cell culture
• mouse model

Objective: To investigate the effects of antiproliferative drugs (anastrozole, methotrexate, and 5-fluorouracil [5-FU]) on the proliferation of endometriotic cells in vitro and in vivo.
Design: Ex vivo study on human endometrial and endometriotic cells in culture; establishment of a murine model using mice implanted with human endometriosis.
Setting: University research center.
Patient(s): Ten patients with ovarian endometrioma, 10 patients with deep infiltrating endometriosis, and 10 patients without endometriosis.
Intervention(s): Stromal and epithelial cells were extracted from endometrial and endometriotic biopsies from patients with endometriosis and from patients without endometriosis. Cells were treated in vitro with anastrozole, methotrexate, progesterone, or 5-FU. Human endometriotic lesions were implanted in nude mice. Mice were treated with 5-FU or phosphate-buffered saline during 4 weeks before sacrifice and extraction of the endometriotic implants.
Main Outcome Measure(s): Stromal and epithelial cell proliferation and pathology score of endometriotic implants.
Result(s): Although anastrozole, methotrexate, and progesterone were ineffective, 5-FU significantly decreased the proliferation of endometriotic cells in vitro and controlled the growth of both cells from ovarian endometrioma and deep infiltrating endometriosis.
Conclusion(s): Considering common features between endometriotic cells and tumor cells, the use of 5-FU could be an option in the management of severe endometriosis. (Fertil Steril (R) 2010;94:1632-8.

• 出版日期2010-10