Advance in Ultrasensitive Detection of MicroRNA

作者：Wang Ziyue; Liu Meng; Zhang Chunyang

来源：Chemical Journal of Chinese Universities, 2017, 38(1): 1-11.

DOI：10.7503/cjcu20160587

• MicroRNA
• Ultrasensitive detection
• Amplification
• Analytical method

MicroRNA( miRNA ) is a class of endogenous noncoding RNA molecule with the length of about 22 nucleotides. MicroRNA may regulate more than 50% of human protein. coding genes, and plays important roles in regulating cell differentiation and other biological processes. The abnormal expression of miRNA is closely related to the occurrence of many diseases including cancers. Therefore, miRNA may function as a potential biomarker for molecular diagnostics. The sensitive detection of miRNA is essential to the early clinical diagnosis and the development of anticancer drugs. Because of the short size, low abundance in total RNA samples and the homologous similarities in miRNA family, the ultrasensitive detection of miRNA remains a great challenge. Recently, many new methods have been developed to improve the detection sensitivity. This review focuses on the advance in miRNA detection methods including colorimetric assay, fluorescent assay, chemiluminescent assay, surface enhanced Raman scattering, single molecules detection and electrochemical detection. In addition, we give a new insight into future development of miRNA assay.

• 出版日期2017-1-10
• 单位山东师范大学