Microtubule-Severing ATPase Spastin in Glioblastoma: Increased Expression in Human Glioblastoma Cell Lines and Inverse Roles in Cell Motility and Proliferation

作者：Draberova Eduarda; Vinopal Stanislav; Morfini Gerardo; Liu Pei S; Sladkova Vladimira; Sulimenko Tetyana; Burns Matthew R; Solowska Joanna; Kulandaivel Kandan; de Chadarevian Jean Pierre; Legido Agustin; Mork Sverre J; Janacek Jiri; Baas Peter W; Draber Pavel; Katsetos Christos D^*

来源：Journal of Neuropathology and Experimental Neurology, 2011, 70(9): 811-826.

DOI：10.1097/NEN.0b013e31822c256d

• Astrocyte
• Brain tumor
• Cell motility
• Glioblastoma
• Glioma
• Microtubule severing
• Spastin

We studied the expression and distribution of the microtubule-severing enzyme spastin in 3 human glioblastoma cell lines (U87MG, U138MG, and T98G) and in clinical tissue samples representative of all grades of diffuse astrocytic gliomas (n = 45). In adult human brains, spastin was distributed predominantly in neurons and neuropil puncta and, to a lesser extent, in glia. Compared with normal mature brain tissues, spastin expression and cellular distribution were increased in neoplastic glial phenotypes, especially in glioblastoma (p < 0.05 vs low-grade diffuse astrocytomas). Overlapping punctate and diffuse patterns of localization were identified in tumor cells in tissues and in interphase and mitotic cells of glioblastoma cell lines. There was enrichment of spastin in the leading edges of cells in T98G glioblastoma cell cultures and in neoplastic cell populations in tumor specimens. Real-time polymerase chain reaction and immunoblotting experiments revealed greater levels of spastin messenger RNA and protein expression in the glioblastoma cell lines versus normal human astrocytes. Functional experiments indicated that spastin depletion resulted in reduced cell motility and higher cell proliferation of T98G cells. To our knowledge, this is the first report of spastin involvement in cell motility. Collectively, our results indicate that spastin expression in glioblastomas might be linked to tumor cell motility, migration, and invasion.

• 出版日期2011-9