科研之友
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摘要
The phytase coding gene (PhyA) of size 1.3 kb isolated from Aspergillus ficuum NRRL 3135 and cloned in Pichia pastoris strain GS115 as recombinant pPICZ alpha A-phytase was used for phytase production. The extracellular phytase activity after optimization with different co-substrates induction strategy in 4 L fermentor (NBS) increased to 13250 U/ml which is 509 fold increases compared to the wild type. This activity was achieved after inducing GS115/pPICZ alpha A-phytase with methanol along with sorbitol as co-feeding in fed batch fermentation at 20 degrees C. The cloned phytase gene will have a potential to produce the thermostable enzyme which could be used in food industry as animals diets.
- 出版日期2014-12
