MPT64 Protein from Mycobacterium tuberculosis Inhibits Apoptosis of Macrophages through NF-kB-miRNA21-Bcl-2 Pathway

作者：Wang Qingmin^*; Liu Shupeng; Tang Ying; Liu Qiuhong; Yao Yongjie

来源：PLos One, 2014, 9(7): e100949.

DOI：10.1371/journal.pone.0100949

MPT64 is one of the secreted proteins from Mycobacterium tuberculosis. Little is known about its role in infection by Mycobacterium tuberculosis. In this study, we demonstrated that MPT64 could dose-dependently inhibit the apoptosis of RAW264.7 macrophages induced by PPD-BCG. Quantitative real-time PCR results showed that the expression of bcl-2 increased in macrophages treated with MPT64 compared with PPD-treated cells. Furthermore, the results provided strong evidence that bcl-2 up-regulation was positively controlled by miRNA-21. Finally, NF-kappa B was identified as the transcription factor for miRNA-21 using a ChIP assay. It can be concluded from our study that MPT64 could inhibit the apoptosis of RAW264.7 macrophages through the NF-kappa B-miRNA21-Bcl-2 pathway.

• 出版日期2014-7-7
• 单位中国人民解放军第二军医大学; 中国人民解放军海军医学研究所