Advances in synthetic dynamic circuits design: using novel synthetic parts to engineer new generations of gene oscillations

作者：Sowa Steven William; Gelderman Grant; Contreras Lydia Maria

来源：Current Opinion in Biotechnology, 2015, 36: 161-167.

DOI：10.1016/j.copbio.2015.08.020

As bioengineering applications expand, the need to design and implement circuits that exhibit dynamic properties increases. In particular, schemes that control precise patterns of gene expression as a function of time are essential for balancing multiple metabolic objectives in natural and synthetic systems. Given that modularity has been an important component of dynamic circuits, recent efforts to improve dynamic circuits have focused on replacing old parts for new components that increase the robustness, stability, and tunability. In this review, we show that incorporation of novel components such as regulatory noncoding RNAs (ncRNAs), promoter-transcription factor pairs, and metabolite sensors have allowed traditional dynamic circuits to obtain more robust functionality and improved dynamic properties.

• 出版日期2015-12