The use of micropatterning to control smooth muscle myosin heavy chain expression and limit the response to transforming growth factor beta 1 in vascular smooth muscle cells

作者：Williams Corin; Brown Xin Q; Bartolak Suki Erzsebet; Ma Hongwei; Chilkoti Ashutosh; Wong Joyce Y^*

来源：Biomaterials, 2011, 32(2): 410-418.

DOI：10.1016/j.biomaterials.2010.08.105

• Micropatterning
• Smooth muscle cell
• Transforming growth factor beta 1
• Arterial tissue engineering

In the healthy artery contractile vascular smooth muscle cells (VSMCs) have an elongated shape and are highly aligned but transition to a synthetic phenotype in culture while additionally becoming well spread and randomly organized Thus controlling VSMC phenotype is a challenge in tissue engineering In this study we investigated the effects of micropatterning on contractile protein expression in VSMCs at low and high passage and in the presence of transforming growth factor beta 1 (TGF beta 1) Micropatterning led to significantly decreased cell area Increased elongation and Increased alignment compared to non-patterned VSMCs independent of passage number In the presence of serum micropatterning led to increased smooth muscle myosin heavy chain (SM-MHC) and alpha-actin expression in low passage VSMCs but had no effect on high passage VSMCs Micropatterning was as effective as TGF beta 1 in up-regulating SM-MHC at low passage however micropatterning limited VSMC response to TGF beta 1 at both low and high passage Investigation of TGF beta receptor 1 revealed higher expression in non-patterned VSMCs compared to patterned at high passage Our studies demonstrate that micropatterning is an I

• 出版日期2011-1