Mini-Tn7 vectors as genetic tools for single copy gene cloning in Acinetobacter baumannii

作者：Kumar Ayush^*; Dalton Courtney; Cortez Cordova Jenny; Schweizer Herbert P

来源：Journal of Microbiological Methods, 2010, 82(3): 296-300.

DOI：10.1016/j.mimet.2010.07.002

• A. baumannii
• Mini-Tn7
• Single-copy gene cloning

Acinetobacter baumannii is an emerging pathogen that causes serious infections with high mortality rates in immunocompromised individuals. Genetic manipulations in this medically-relevant pathogen are limited by the paucity of molecular tools. In this study, we show the application of the mini-Tn7-based single copy insertion system in A. baumannii. Mini-Tn7 elements are known to integrate at a naturally evolved, therefore presumably neutral location (intergenic region) downstream of the glmS gene (glucosamine-fructose-6-phosphate aminotransferase) in Gram-negative bacteria. We identified the site of insertion of mini-Tn7 in A. baumannii and demonstrated application of this useful cloning tool by inserting the gfp gene into the chromosome. Our work shows that mini-Tn7 elements are useful tools for genetic studies in this important pathogen.

• 出版日期2010-9