Discrimination of young and mature leaves of Melicope ptelefolia using H-1 NMR and multivariate data analysis

作者：Shuib Nor Hassifi; Shaari Khozirah; Khatib Alfi^*; Maulidiani; Kneer Ralf; Zareen Seema; Raof Salahudin Mohd; Lajis Nordin Hj; Neto Victor

来源：Food Chemistry, 2011, 126(2): 640-645.

DOI：10.1016/j.foodchem.2010.10.043

• Metabolomics
• Multivariate data analysis
• Principle component analysis
• NMR
• Melicope ptelefolia

The 'Ulam', a traditional Malay dish, are plants that can be eaten raw, as a form of local salad. The shoots and young leaves of Melicope ptelefolia are among the popular species, believed to be high in nutritional and medicinal values. The metabolomic fingerprinting analysis of the ethanolic extracts of leaves of M. ptelefolia was carried out using H-1 Nuclear Magnetic Resonance (NMR) spectroscopy and multivariate data analysis in order to differentiate young and mature leaves and to evaluate the variation of their chemical composition. Principle component analysis (PCA) of the H-1 NMR spectra showed a clear discrimination between the young and mature leaves extracts by PC3 and PC4. The compounds responsible for the differentiation were identified by comparison of H-1 NMR chemical shifts and qualitative HPLC. The young leaves were found to be richer in fatty acids and the levels of the three marker compounds, p-O-geranylcoumaric acid, 2,4,6-trihydroxy-3-geranylacetophenone and 2,4,6-trihydroxy-3-prenylacetophenone, were clearly higher. The mature leaves contain higher levels of sugars and glycosidic components.

• 出版日期2011-5-15