Microencapsulated Schwann cell transplantation inhibits P2X3 receptor expression in dorsal root ganglia and neuropathic pain

作者：Zhang, Ya-Ling; Chen, De-Jian; Yang, Bao-Lin; Liu, Tao-Tao; Li, Jia-Juan; Wang, Xiu-Qi; Xue, Guo-Yong; Liu, Zeng-Xu^*

来源：Neural Regeneration Research, 2018, 13(11): 1961-1967.

DOI：10.4103/1673-5374.238715

• nerve regeneration
• neuropathic pain
• peripheral nerve injury
• sciatic nerve
• microencapsulation
• Schwann cells
• P2X3 receptor
• dorsal root ganglion
• chronic constriction injury
• cell transplantation
• neural repair
• neural regeneration

Schwann cell transplantation is a promising method to promote neural repair, and can be used for peripheral nerve protection and myelination. Microcapsule technology largely mitigates immune rejection of transplanted cells. We previously showed that microencapsulated olfactory ensheathing cells can reduce neuropathic pain and we hypothesized that microencapsulated Schwann cells can also inhibit neuropathic pain. Rat Schwann cells were cultured by subculture and then microencapsulated and were tested using a rat chronic constriction injury (CCI) neuropathic pain model. CCI rats were treated with Schwann cells or microencapsulated Schwann cells and were compared with sham and CCI groups. Mechanical withdrawal threshold and thermal withdrawal latency were assessed preoperatively and at 1, 3, 5, 7, 9, 11 and 14 days postoperatively. The expression of P2X3 receptors in L4-5 dorsal root ganglia of the different groups was detected by double-label immunofluorescence on day 14 after surgery. Compared with the chronic constriction injury group, mechanical withdrawal threshold and thermal withdrawal latency were higher, but the expression of P2X3 receptors was remarkably decreased in rats treated with Schwann cells and microencapsulated Schwann cells, especially in the rats transplanted with microencapsulated Schwann cells. The above data show that microencapsulated Schwann cell transplantation inhibits P2X3 receptor expression in L4-5 dorsal root ganglia and neuropathic pain.

• 出版日期2018-11
• 单位南昌大学