Novel kinetic spectrophotometric method for estimation of certain biologically active phenolic sympathomimetic drugs in their bulk powders and different pharmaceutical formulations

作者：Omar Mahmoud A; El Din Khalid M Badr; Salem Hesham; Abdelmageed Osama H^*

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2018, 193: 310-317.

DOI：10.1016/j.saa.2017.12.045

• Spectrophotometry
• Sympathomimetic drugs
• Folin-Ciocalteu
• Kinetic determination

A simple, selective and sensitive kinetic spectrophotometric method was described for estimation of four phenolic sympathomimetic drugs namely; terbutaline sulfate, fenoterol hydrobromide, isoxsuprine hydrochloride and etilefrine hydrochloride. This method is depended on the oxidation of the phenolic drugs with Folin-Ciocalteu reagent in presence of sodium carbonate. The rate of color development at 747-760 nm was measured spectrophotometrically. The experimental parameters controlling the color development were fully studied and optimized. The reaction mechanism for color development was proposed. The calibration graphs for both the initial rate and fixed time methods were constructed, where linear correlations were found in the general concentration ranges of 3.65 x 10(-6)-2.19 x 10(-5) mol L-1 and 2-24.0 mu g mL(-1) with correlation coefficients in the following range 0.9992-0.9999, 0.9991-0.9998 respectively. The limits of detection and quantitation for the initial rate and fixed time methods were found to be in general concentration range 0.109-0.273, 0.363-0.910 and 0.210-0.483, 0.700-1.611 mu g mL(-1) respectively. The developed method was validated according to ICH and USP 30 -NF 25 guidelines. The suggested method was successfully implemented to the estimation of these drugs in their commercial pharmaceutical formulations and the recovery percentages obtained were ranged from 97.63% +/- 137 to 100.17% +/- 0.95 and 9729% +/- 0.74 to 100.14 +/- 0.81 for initial rate and fixed time methods respectively. The data obtained from the analysis of dosage forms were compared with those obtained by reported methods. Statistical analysis of these results indicated no significant variation in the accuracy and precision of both the proposed and reported methods.

• 出版日期2018-3-15