Accumulation of Activated Invariant Natural Killer T Cells in the Tumor Microenvironment after alpha-Galactosylceramide-Pulsed Antigen Presenting Cells

作者：Nagato Kaoru; Motohashi Shinichiro^*; Ishibashi Fumihiro; Okita Kohsuke; Yamasaki Kazuki; Moriya Yasumitsu; Hoshino Hidehisa; Yoshida Shigetoshi; Hanaoka Hideki; Fujii Shin ichiro; Taniguchi Masaru; Yoshino Ichiro; Nakayama Toshinori

来源：Journal of Clinical Immunology, 2012, 32(5): 1071-1081.

DOI：10.1007/s10875-012-9697-9

• Invariant NKT cell
• antigen presenting cell
• immunotherapy
• tumor infiltrating lymphocyte
• non-small cell lung cancer

The intravenous administration of alpha-Galactosylceramide (alpha-GalCer)-pulsed antigen presenting cells (APCs) is well tolerated and the increased IFN-gamma producing cells in the peripheral blood after the treatment appeared to be associated with prolonged survival. An exploratory study protocol was designed with the preoperative administration of alpha-GalCer-pulsed APCs to clarify the mechanisms of these findings, while especially focusing on the precise tumor site. %26lt;br%26gt;Patients with operable advanced lung cancer received an intravenous injection of alpha-GalCer-pulsed APCs before surgery. The resected lung and tumor infiltrating lymphocytes (TILs) as well as peripheral blood mononuclear cells were collected and the invariant NKT (iNKT) cell-specific immune responses were analyzed. %26lt;br%26gt;Four patients completed the study protocol. We observed a significant increase in iNKT cell numbers in the TILs and augmented IFN-gamma production by the alpha-GalCer-stimulated TILs. %26lt;br%26gt;The administration of alpha-GalCer-pulsed APCs successfully induced the dramatic infiltration and activation of iNKT cells in the tumor microenvironment.

• 出版日期2012-10