Development of a biomimetic enzyme-linked immunosorbent assay based on molecularly imprinted polymers on paper for the detection of carbaryl

作者：Zhang, Can^*; Cui, Hanyu; Han, Yufeng; Yu, Fangfang; Shi, Xiaoman

来源：Food Chemistry, 2018, 240: 893-897.

DOI：10.1016/j.foodchem.2017.07.109

• Molecularly imprinted polymer
• Biomimetic enzyme-linked immunosorbent assay
• Paper
• Carbaryl
• Enzyme-linked immunosorbent assay

A biomimetic enzyme-linked immunosorbent assay (BELISA) which was based on molecularly imprinted polymers on paper (MIPs-paper) with specific recognition was developed. As a detector, the surface of paper was modified with gamma-MAPS by hydrolytic action and anchored the MIP layer on gamma-MAPS modified-paper by copolymerization to construct the artificial antibody Through a series of experimentation and verification, we successful got the MIPs-paper and established BELISA for the detection of carbaryl. The development of MIPs-paper based on BELISA was applied to detect carbaryl in real samples and validated by an enzyme-linked immunosorbent assay (ELISA) based on anti-carbaryl biological antibody. The results of these two methods (BELISA and ELISA) were well correlated (R-2 = 0.944). The established method of MIPs-paper BELISA exhibits the advantages of low cost, higher stability and being re-generable, which can be applied as a convenient tool for the fast and efficient detection of carbaryl.

• 出版日期2018-2-1
• 单位江苏大学