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摘要
In order to explore whether endophytic fungi of Casuarina equisetifolia L. are involved in allelopathy, we investigated their diversity and composition of their metabolites. By Illumina Miseq high-throughput sequencing platform, a total of 318,972 sequences were obtained from 9 root samples of C. equisetifolia of different ages, which included 11 phyla, 32 families and 32 genera. Four fungi were isolated from the roots by the traditional method and identified by biolog microbial automatic analysis system as Aspergillus aculeatus lzuka, Penicillium melinii Thom, Neosartorya fisccheri (Wehmer) Malloch & Cain BGA and Penicillium solitum Westling BGB. Pyrogallic acid, dodecanoic acid, palmitic acid and stearic acid, 2,4-Di-tert-butylphenol, 1,2,3,4-butanetetrol and globulol and other organic compounds were identified in the fermentation broths of four endophytic fungi by GC-MS analysis. Among them, 2, 4-Ditert-butylphenol, 1,2,3,4-butanetetrol and globulol were also found in soil, root and litter extract. These results imply that root-endophytic fungi are involved in the synthesis of substances that affect the host plants. In bioassays, the fermentation broth of the four root-endophytic fungi significantly inhibited the seed germination of Thespesia lampas L. and Calophyllum inophyllum L.
- 出版日期2018-11
- 单位海南师范大学
