DETECTION OF HUMAN PAPILLOMAVIRUS TYPE-16 DNA-SEQUENCES IN ARCHIVAL CERVICAL TISSUES BY THE POLYMERASE CHAIN-REACTION

作者：HORDING U^*; DAUGAARD S; IVERSEN AKN; BOCK JE; PHILIP J

来源：Archives of Pathology & Laboratory Medicine, 1992, 116(6): 632-634.

We have evaluated the polymerase chain reaction for the detection of viral DNA sequences in paraffin-embedded archival tissues. In 63 frozen cervical biopsy specimens that were taken from premalignant and invasive lesions, Southern blotting detected human papillomavirus (HPV) type 16 DNA in 28 (44%) of the samples. In the polymerase chain reaction analysis of the formalin-fixed, paraffin-embedded mirror biopsy specimens, 46 (73%) of the tissues were found to be positive for HPV type 16. In three Southern blotting-positive cases, the DNA of the paraffin-embedded sections was too scant or too degraded to allow the detection of HPV DNA by the polymerase chain reaction. In 21 Southern blotting-negative cases, HPV type 16 DNA could be demonstrated in the archival sections by the polymerase chain reaction technique-a sensitivity improvement of more than 80% over the standard method of HPV detection in tissues.

• 出版日期1992-6
• 单位河北医科大学