Apoptotic toxicity of destruxin B in human non-Hodgkin lymphoma cells

作者：Chao Pin Zhir; Chin Yi Ping; Hsu I Uen; Liu Cheng Ming; Yu Ya Chi; Leung Ting Kai; Lee Yuarn Jang; Chen Chien Ho^*; Lin Yung Feng

来源：Toxicology in Vitro, 2013, 27(6): 1870-1876.

DOI：10.1016/j.tiv.2013.05.016

• Destruxin B
• Non-Hodgkin lymphoma
• Mitochondrial membrane potential
• Apoptosis
• Death receptor pathway

Destruxins are fungal toxins used as insecticides. Recent reports demonstrated the potential anti-cancer activities of destruxin B (DB). This study is to discover the effects of DB in lymphoma. Flow cytometry and Western blotting were used to analyze apoptosis and protein expression, respectively, in Toledo human non-Hodgkin lymphoma cells in response to DB. Administration of DB, induced apoptosis via death receptor pathway activating Fas associated death domain (FADD), caspase 8 and caspase 3, and suppressed the cell growth. In addition, DB alterated mitochondrial membrane potential by increasing the expressions of tBid and Bax, but decreasing the levels of Bcl-2, resulting in the release of apoptosis-inducing factor (AIF). In conclusion, apoptosis of human non-Hodgkin lymphoma cells in response to DB is induced through the death receptor pathway and involves an alteration of the mitochondrial membrane potential. These findings may aid the development of novel treatment of non-Hodgkin lymphoma.

• 出版日期2013-9