Molecular typing and antimicrobial susceptibility profiles of Campylobacter jejuni isolates from north China

作者：Zhang Maojun; Gu Yixin; He Lihua; Ran Lu; Xia Shengli; Han Xiaosheng; Li Hongxing; Zhou Haijian; Cui Zhigang; Zhang Jianzhong^*

来源：Journal of Medical Microbiology, 2010, 59(10): 1171-1177.

DOI：10.1099/jmm.0.022418-0

To obtain the genotype and antimicrobial susceptibility profiles of Campylobacter jejuni isolates from north China, 93 C. jejuni isolates (56 isolates from patients with diarrhoea, 7 isolates from Guillain-Barre syndrome patients and 30 isolates from chicken stools) were selected for multilocus sequence typing (MLST), PFGE and drug resistance testing A total of 49 sequence types (STs) were identified from the entire panel of 93 C. jejuni isolates. Fifty-six isolates belonged to 14 clonal complexes, while 37 isolates could not be assigned to any known clonal complex. The most frequently observed clonal complexes were ST-21 (11 isolates), ST-353 (10 isolates) and ST-443 (6 isolates). Fifty-three PFGE Smal patterns were identified among 93 isolates. No erythromycin-, gentamicin- or streptomycin-resistant isolates were found among the 44 strains isolated in 2008. Resistance to nalidixic acid. levofloxacin and ciprofloxacin was observed in 100% (44/44) of the tested isolates. This study has shown the genetic characteristics of C. jejuni isolates in north China In addition, overlapping clonal groups were defined by both MLST and PFGE for C. jejuni human and chicken isolates.

• 出版日期2010-10
• 单位中国疾病预防控制中心; 中国疾病预防控制中心传染病预防控制所